T4 DNA Polymerase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M0203S |
T4 DNA Polymerase |
150 units ( 3000 units/ml ) | - | Unavailable in your region | |
M0203L |
T4 DNA Polymerase |
750 units ( 3000 units/ml ) | - | Unavailable in your region |
T4 DNA Polymerase
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer.
- Gap filling (no strand displacement activity)
- Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
- Lacks 5’ —> 3’ exonuclease activity
- Probe labeling using replacement synthesis
- Singe-strand deletion subcloning
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Product Information
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.
Product Source
Purified from a strain of E. coli that carries the T4 DNA Polymerase gene.- This product is related to the following categories:
- DNA Manipulation
- This product can be used in the following applications:
- Blunting,
- Polymerases for DNA Manipulation,
- PCR
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Protocols, Manuals & Usage
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Citations & Technical Literature
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The supporting documents available for this product can be downloaded below.