Product Class: Other

Anti-MBP Magnetic Beads

This product will be discontinued on December 15, 2024. An alternative product is the Amylose Magnetic Beads (NEB #E8035S).

Product Introduction

An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins. 

  • Monoclonal anti-MBP is covalently coupled to 1 µm nonporous superparamagnetic particles through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the immunomagnetic isolation of MBP-fusion proteins from cell culture extracts
  • Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein
  • Binding capacity of 5 μg MBP5*-paramyosin ΔSal fusion protein/mg Anti-MBP Magnetic Beads
  • Quick, small-scale purification of MBP-fusion proteins enables higher throughput and efficiency
Catalog # Size Concentration
E8037S 10.0 mg 10 mg/ml

Product Information

Description

An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins. Monoclonal anti-MBP is covalently coupled to 1 µm nonporous paramagnetic particles through a linkage that is stable and leak resistant over a wide pH range. This permits immunomagnetic isolation of MBP-fusion proteins from cell culture extracts. Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

This product is related to the following categories:
Magnetic Beads and Racks Products
This product can be used in the following applications:
High-throughput cloning and automation solutions

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.1% BSA
0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Support Matrix

1 μm nonporous superparamagnetic microparticle

Binding Capacity

5 μg MBP-paramyosin Dsal fusion protein / mg Anti-MBP beads.

Shipping Notes

  • 4°C

Protocols, Manuals & Usage

Protocols

  1. Isolation of MBP-fusion protein using Anti-MBP Magnetic Beads

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Do I need to block the beads or are they pre-blocked?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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