NEBNext® Low-bias Small RNA Library Prep Kit
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E3420S |
NEBNext Low-bias Small RNA Library Prep Kit |
24 Assays | - | Unavailable in your region | |
E3420L |
NEBNext Low-bias Small RNA Library Prep Kit |
96 Assays | - | Unavailable in your region |
NEBNext® Low-bias Small RNA Library Prep Kit
Catalog #E3420
Product Introduction
When seeing the full picture of small RNA species in a sample matters, there is only one library prep kit that truly delivers on the promise of being low bias. The NEBNext Low-bias Small RNA Library Prep Kit was designed to generate libraries that accurately reflect the relative proportions of RNA species present in a sample. The straightforward gel-free protocol with a wide input range makes this kit the fastest high-sensitivity kit for small RNA library prep on the market.
- Lowest bias of all commercially available kits
- Biologically relevant input range (1,000 ng – 0.5 ng Total RNA; 5 ng – 0.05 ng Enriched small RNA)
- Faster, single-day workflow
- Multiplexable, up to 480 LV Unique Dual Index primer pairs (available separately)
- 18-month shelf life
Note: The original NEBNext Small RNA Kits (NEB #E7300, E7580, E7560, E7330) are still available for purchase, although NEB recommends the NEBNext Low-bias Small RNA Library Prep Kit for enhanced accuracy, library quality, and improved workflow.
- Product Information
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- FAQs & Troubleshooting
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Product Information
Description
The NEBNext Low-bias Small RNA Library Prep Kit minimizes biased representation of small RNA species, more accurately reflecting the number and proportion of unique small RNAs present. NEB's next-generation kit does this via a randomized splint ligation-based approach (see below), which captures small RNAs (<120 nt) with a 5' phosphate and 3' hydroxyl group. Species identified include microRNAs (miRNAs) from a variety of samples (e.g., human, plant), transfer RNAs (tRNAs) and tRNA-derived fragments, small nucleolar RNAs (snoRNAs), and piwi-interacting RNAs (piRNAs). This kit is designed for the preparation of Illumina®-compatible small RNA sequencing libraries.
Benefits:
- Analyze all RNA species present, without library prep-generated bias
- Simplify and streamline your library prep workflow (~3.5 hours), saving time
- Derive insights from biologically relevant input amounts
- Plan ahead with a 18-month shelf life
- Learn a single protocol for standard and 2'-O-methylated samples
- Expand your insights with up to 480 compatible UDI primer pairs, available separately
The NEBNext Low-bias Small RNA Library Prep Kit was developed following the 2020 publication of original research work by NEB scientists in Nucleic Acids Research. The article, "A low-bias and sensitive small RNA library preparation method using randomized splint ligation" was authored by Sean Maguire, Greg Lohman, and Shengzi Guan. Several protocol enhancements have improved the kit to outperform the originally published methods.
This kit is compatible with NEBNext LV Unique Dual Index Primer Pairs Sets 1-5; see neb.com/oligos for more details.
Each kit component must pass rigorous quality control standards and, for each new lot, the entire set of reagents is functionally validated together by construction of an indexed library and sequencing on an Illumina sequencer.







More data supporting the claims made about the NEBNext Low-bias Small RNA Library Prep Kit can be found on the E3420 Supplemental Data Page.
- This product is related to the following categories:
- Small RNA Library Preparation ,
- Next Generation Sequencing Library Preparation,
Properties & Usage
Related Products
Companion Products
Protocols, Manuals & Usage
Protocols
Manuals
Tools & Resources
Selection Charts
FAQs & Troubleshooting
FAQs
- What is the formulation of 0.1X TE provided with the NEBNext Low-bias Small RNA Library Prep Kit?
- Are libraries generated with the NEBNext Low-bias Small RNA Library Prep Kit compatible with paired-end Illumina sequencing?
- What are the sequences needed to trim the NEBNext Low-bias Small RNA libraries?
- Are custom sequencing primers needed for sequencing NEBNext Low-bias Small RNA libraries?
- When is the RT primer annealed during the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?
- What should I do if my RNA does not have 5' monophosphate and 3' OH ends before starting the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?
- Is it possible to prevent abundant, unwanted RNAs from being included in NEBNext Low-bias Small RNA libraries (NEB #E3420)?
- Can I use total RNA as input into NEBNext Low-bias Small RNA Library Prep Kit (NEB #E3420), or do I need to isolate or enrich specifically for small RNAs?
- What are typical yields for a NEBNext Low-bias Small RNA library (NEB #E3420)?
- How important is the volume of isopropanol in the NEBNext Low-bias Small RNA Library Prep Kit (NEB #E3420) protocol?
- Can I do a gel size selection on the final libraries generated using the NEBNext Low-bias Small RNA Library Prep Kit (NEB #E3420)?
- What is the required RIN for Total RNA to be used in NEBNext Low-bias Small RNA Library (NEB #E3420) generation?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.100mM DTT
0.1X TE
NEBNext® Ultra II Q5® Master Mix
NEBNext® Sample Purification Beads
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)
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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)
-
NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)
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Monarch® Spin RNA Isolation Kit (Mini)
The supporting documents available for this product can be downloaded below.