NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
Note that additional index primers for multiplexing are provided with kit NEB #E7580 and NEB #E7560.
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
Minimized adaptor-dimer contamination
High yields
Input RNA can be Total RNA
Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
The novel NEBNext® Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext® Small RNA Library Prep Set for Illumina® (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing. Additional index primers for multiplexing are included in kits NEB #E7580 and NEB #E7560.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of indexed small RNA libraries on the Illumina sequencing platform.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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NEW ENGLAND BIOLABS®, LongAmp®, NEBNext® and QUICK-LOAD® are registered trademarks of New England Biolabs, Inc.
AGENCOURT® and AMPure® are registered trademarks of Beckman Coulter, Inc. Agilent® and Bioanalyzer® are registered trademarks of Agilent Technologies, Inc. Illumina® is a registered trademark of Illumina, Inc. CORNING®, COSTAR® and SPIN-X® are registered trademarks of Corning, Inc. NOVEX®, FIRSTCHOICE® and SYBR® are registered trademarks of Life Technologies, Inc. FLASHPAGE™ is a registered trademark of Life Technologies, Inc. Pellet Pestle® is a registered trademark of Kimble Kontes Asset Management, Inc. Qiagen® and QIAQUICK® are registered trademarks of The Qiagen Group.