Product Class: Kit

Quick Blunting™ and Quick Ligation™ Kits

This product will be discontinued on December 15, 2024. Individual products are still available: Quick Blunting™ Kit (NEB #E1201) and Quick Ligation™ Kit (NEB #M2200).

Product Introduction

NEB offers the Quick Blunting™ and Quick Ligation Kits™ together as a bundle at a value price.

  • The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. 
  • The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
Catalog # Size Concentration
E0542S 20.0 reactions
E0542L 100.0 reactions

Featured Videos

View Video Library
  • DNA Ligase

    DNA Ligation

  • DNABluntingVideo_thumb

    DNA Blunting Tutorial

  • PhosphorylationVideo_thumb

    The Mechanism of DNA Phosphorylation

Product Information

Description

NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
This product is related to the following categories:
DNA Ligases Products,
This product can be used in the following applications:
Phosphorylation (Kinase),
Blunting,
Cloning Ligation,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E0542S     -20    
      Quick Ligation™ Kit M2200S -20 1 x 30 reactions Not Applicable
      Quick Ligase M2200SVIAL -20 1 x 0.03 ml Not Applicable
      Quick Ligation Reaction Buffer B2200SVIAL -20 1 x 1 ml 2 X
      Quick Blunting™ Kit E1201S -20 1 x 20 reactions Not Applicable
      Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable
      10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X
      Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM
  • E0542L     -20    
      Quick Ligation™ Kit M2200L -20 1 x 150 reactions Not Applicable
      Quick Ligase M2200LVIAL -20 1 x 0.15 ml Not Applicable
      Quick Ligation Reaction Buffer B2200SVIAL -20 4 x 1 ml 2 X
      Quick Blunting™ Kit E1201L -20 1 x 100 reactions Not Applicable
      Blunting Enzyme Mix M1201AAVIAL -20 1 x 0.125 ml Not Applicable
      10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X
      Deoxynucleotide Solution Mix (1 mM) N1201AAVIAL -20 1 x 0.5 ml 1 mM

Properties & Usage

Product Notes

  1. Please note storage conditions for individual products.

Protocols, Manuals & Usage

Protocols

  1. Protocol for the Quick Blunting Kit (E1201)
  2. Transformation Protocol
  3. Quick Ligation Protocol (M2200)

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. Will the PreCR Repair Mix blunt the ends of the DNA?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In