Product Class: Kit

Endoglycosidase Reaction Buffer Pack

Product Introduction

The Endoglycosidase Reaction Buffer Pack contains four buffers, 10X Glycobuffer 2, 10X Glycobuffer 3, 10X Glycoprotein Denaturing Buffer and 10% NP-40, most commonly used in endoglycosidase reactions.

  • At a 1X concentration these reaction buffers assure optimal activity of the enzymes
  • Reaction buffers have no detectable endoglycosidase, exoglycosidase or protease contaminating activities
  • Optimal stability for up to 24 months
Catalog # Size Concentration
B0701S 4.0 ml

Product Information

Description

New England Biolabs supplies a 10X reaction buffer with all of its enzymes.

1X GlycoBuffer 2:
50 mM Sodium Phosphate (pH 7.5 @ 25°C)

1X GlycoBuffer 3:
50 mM Sodium Acetate (pH 6.0 @ 25°C)

1X Glycoprotein Denaturing Buffer
0.5% SDS, 40 mM DTT

1% NP-40
This product is related to the following categories:
Glycobiology Buffers & Diluents Products,
Endoglycosidases Products,
Buffers Products,
This product can be used in the following applications:
Protein Digestion

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • B0701S     -20    
      GlycoBuffer 2 B3704SVIAL -20 1 x 1 ml 10 X
      Glycoprotein Denaturing Buffer B1704SVIAL -20 1 x 1 ml 10 X
      NP-40 B2704SVIAL -20 1 x 1 ml 10 %
      GlycoBuffer 3 B1720SVIAL -20 1 x 1 ml 10 X

Properties & Usage

Product Notes

  1. Buffer Compositions

    1X GlycoBuffer 2:
    50 mM Sodium Phosphate
    pH 7.5 @ 25°C

    1X GlycoBuffer 3:
    50 mM Sodium Acetate
    pH 6.0 @ 25°C

    1X Glycoprotein Denaturing Buffer:
    0.5% SDS, 40 mM DTT

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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