PCR clean-up and gel extraction from small samples

 

NucleoSpin® Gel and PCR Clean-up XS – Small, yet powerful!

The NucleoSpin® Gel and PCR Clean-up XS procedure is the easiest way to concentrate and purify DNA fragments from agarose gels as well as for direct purification of PCR products. The new XS kit contains specialized XS columns to ensures highly efficient recovery of DNA fragments with even very small elution volumes of only 6 µL. The special XS column design funnels every drop of elution buffer directly through the specialized silica membrane thereby achieving maximum concentration of your sample. Our new NucleoSpin® Gel and PCR Clean-up XS kit will of course also reliably remove primers, enzymes and free dNTPs. It recovers high DNA yields and will provide you with pure, high quality DNA - ready-to-use for PCR, arrays, ligation, sequencing, etc.

 

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Workflow video

 

Technical data

Concentrated clean up with small elution volumes from PCR reaction mixtures and agarose gels

Application	PCR clean up and gel extraction, Sample concentration
Target	Clean up
CE certified	No, research use only
Technology	Silica membrane technology
Brand	NucleoSpin
Format	XS prep
Package unit	10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling	Vacuum, centrifugation
Automated use	No
Sample material	Agarose gel, PCR reaction mixture
Sample amount	< 200 µL PCR reaction mixture, < 200 mg agarose gel
Fragment size	50 bp−approx. 20 kbp
Typical recovery	75−95 %
Theoretical binding capacity	5 µg
Typical purity A260/A280	1.8–1.9
Elution volume	6-12 µL
Preparation time	15 min/6 preps
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing
Storage temperature	18−25 °C
Shelf life (from production)	24 Months
Hazardous material	No

 

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