Principle

The fastest way to desalt and concentrate DNA after your NucleoBond® plasmid preparations

NucleoSnap® Finisher Midi / Maxi kits supersede the commonly used and time consuming isopropanol precipitation and centrifugation steps by allowing the rapid DNA precipitation and concentration of large volumes under vacuum.

DNA is precipitated by Precipitation Buffer FB and filtered by the specially designed matrix of the novel NucleoSnap® Finisher Columns, followed by a washing step with ethanolic Wash Buffer A4 to remove salts and impurities.

To enable the use of a table top centrifuge, the NucleoSnap® Finisher Column is composed of an upper funnel component which can be removed from the lower Mini spin column part by a simple break action. Then the Mini spin column can be centrifuged in a standard table top centrifuge to remove residual ethanol. 

Afterwards, plasmid DNA can be eluted in supplied endotoxin-free H2O-EF. The applied elution volume can be adjusted from 100 μL to 1.5 mL according to the expected amount of plasmid DNA to ensure optimal yield and concentration.

NucleoSnap® Finisher Midi / Maxi procedure

Applications

• Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography
• Typical downstream applications: transfection, in-vitro transcription, automated or manual sequencing, cloning, hybridization,
  and PCR