Time-saving processing of 24 samples in parallel

• Parallel processing of silica columns (e.g., NucleoSpin® and NucleoSnap) for nucleic acid purification
• Fast processing of large sample volumes 

Principle/Procedure

The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns (e.g., NucleoSpin® columns, NucleoSnap columns) can be attached to the upper luer slots of the vacuum manifold. Binding and washing steps of the NucleoSpin® or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling timesaving procedures (NucleoSpin® products) and processing of large sample volumes (NucleoSnap products).

 
NucleoVac 24 Vacuum Manifold setup

 
(1) Rubber closing plug
(2) Tubing connection
(3) Upper opening

(4) NucleoSpin® column
(5) Luer opening
(6) NucleoVac Mini Adapter
(7) NucleoVac Valves
(8) Luer plug

Close the lower threaded opening of the NucleoVac 24 Vacuum Manifold with the rubber closing plug (1) . Screw in the tubing connection (2) in the upper opening (3) manually. Do not use a wrench as the conical screw thread might crack if the connection is over-tightened. Attach tube (not included in the product).

For processing NucleoSpin® (4) or NucleoSnap columns with the NucleoVac 24 Vacuum Manifold, insert NucleoSpin® or NucleoSnap columns into the Luer openings (5), (a).

To avoid cross-contamination, it is recommended to use disposable NucleoVac Mini Adapters (6), (b). For processing samples with different flow rates (e.g., large volume samples), it is recommended to use NucleoVac Valves (7) for maintaining constant vacuum pressure throughout the whole procedure (c). Close unused positions with Luer plugs (8), (d).