Principle

Blood sample storage card for subsequent DNA extraction

Use of liquid blood samples always implicates storage and stability problems whenever the blood is not directly used as starting material for DNA extraction after blood withdrawal. Long-term storage of liquid whole blood requires freezer capacity (-80 °C) as DNA has limited stability in whole blood at any higher temperatures. Especially if shipping of blood samples is required, it is difficult to guarantee maintenance of the cold chain. Nowadays downstream applications and detection systems are significantly improved in terms of sensitivity – thus less DNA is required for the downstream applications and a single blood spot includes sufficient DNA for genotyping. NucleoCard® cards guarantee long-term storage of blood by a sophisticated impregnation of the specialized filter paper. This prevents DNA damage and degradation upon storage. Genomic DNA isolated from blood samples stored on NucleoCard® cards at room temperature for over 3 years (and counting) has been successfully amplified by PCR.

Procedure
(1) Load up to 200 µl blood sample onto collection circles
(2) Let the card dry for 30–60 min and store at 18–25 °C.
(3) For DNA extraction punch out 3 mm disk and purify DNA with a reliable DNA purification kit, for example NucleoSpin® Tissue

Features

* Custom configurations available on request