Principle

The isolation of genomic DNA (gDNA) from the majority of organs is a challenging task.  Organs are composed of different tissues with various types of cells and matrices.  Large molecules such as collagens and proteoglycans can impede lysis and even impair downstream enzymatic reactions (e.g., PCR). The new NucleoSpin® DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.

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Applications*

Kit for the isolation of genomic DNA from tail and ear clippings, organs, and cells
 

Application data

Time saving procedure

The duration of the procedure (incl. lysis) was compared to common extraction methods (competitors S and Q). Fastest gDNA isolation was carried out with NucleoSpin® DNA RapidLyse (MN).

Outstanding DNA Yields

After sample lysis for one hour and subsequent DNA extraction, same amounts of independent eluates were subjected to gel electrophoresis. Superior yields of high molecular weight gDNA could be extracted with NucleoSpin® DNA RapidLyse (MN) in comparison to competitors S and Q.

Highest cost-efficiency

The average price/prep of NucleoSpin® DNA RapidLyse was compared to prices from other DNA purification products. The NucleoSpin® DNA Rapid-Lyse is the most cost efficient product for gDNA isolation in comparison to competitors R, Q, T, AJ, Z, and S.

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* Kits to be used for research purposes only