Principle

Low profile, 0.1 ml wells, polypropylene, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler 480®

This Roche Style plate is designed to achieve optimised assay conditions on the Roche LightCycler® 480.

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

Features

• Optimised for use with the Roche LightCycler® 480
• Recommended for low volume PCR
• Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
• Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
• Compatible with standard multichannel pipettes
• <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
• Subject to a strict QC procedure which includes a visual check and leak testing of every tube
• White wells give optimum signal-to-noise ratio when using fluorescent-based assays
• Raised grid reference to aid well and sample identification
• Available barcoded
• Combi packs available with qPCR Seal (0560)

Alternatively, the FrameStar® 2-component PCR plate range offers a plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss. Take a look here.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Please be aware the barcoding products have a minimum order quantity of 10 boxes.

Technical Specifications