Product Class: Other

Unstained Protein Standard, Broad Range (10-200 kDa)

Product Introduction

The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 or other commercially available, ready-to-use protein stains. 

  • Allows accurate molecular weight determination when performing SDS-PAGE analysis.
  • Direct loading, additional loading buffer and heat incubation not required
  • Recombinant proteins with no detectable protease contaminating activities
  • Optimal stability for up to 24 months

 

Catalog # Size Concentration
P7717S 150 gel lanes
P7717L 750 gel lanes

Product Information

Description

The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 (1,2) or other commercially available, ready-to-use protein stains. This standard allows accurate molecular weight determination when performing SDS-PAGE analysis.



Unstained Protein Standard, Broad Range (10–200 kDa) 10–20% Tris-glycine SDS-PAGE Gel


This product is related to the following categories:
Protein Standards,
Markers & Ladders,
Protein Standards,
This product can be used in the following applications:
Protein Standards,
Protein Analysis Tools

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • P7717S     -20    
      Unstained Protein Standard, Broad Range (10-200 kDa) P7717SVIAL -20 1 x 150 gel lanes Not Applicable
  • P7717L     -20    
      Unstained Protein Standard, Broad Range (10-200 kDa) P7717SVIAL -20 5 x 150 gel lanes Not Applicable

Properties & Usage

Storage Buffer

62.5 mM Tris-phosphate
1 mM EDTA
2% SDS
10 mM DTT
1 mM NaN3
0.01% bromophenol blue
33% Glycerol
pH 7.5 @ -20°C

Features

  • Allows accurate molecular weight determination when performing SDS-PAGE analysis.
  • Direct loading, additional loading buffer and heat incubation not required

Product Notes

  1. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
  2. To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
  3. It is not necessary to pre-heat the unstained protein standard prior to loading on a gel.
  4. Recommended mini-gel load: 3 μl.
  5. Apparent molecular weight values for prestained proteins can be different when run on different gel types and percentages.

References

  1. Laemmli, UK (1970). Nature. 227, 680.
  2. Sambrook, J., Fitsch, EF. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Using the Unstained Protein Standard, Broad Range (10-200 kDa) (P7717) for an SDS-PAGE Mini Gel

FAQs & Troubleshooting

FAQs

  1. How does the Unstained Protein Standard, Broad Range differ from NEB’s previous Unstained Protein Standard?
  2. What is the protein concentration of the proteins used in the Unstained Protein Standard, Broad Range?
  3. Can the Unstained Protein Standard, Broad Range be used as a precise standard for determining the exact molecular weight of a protein of interest?
  4. Is the Unstained Protein Standard, Broad Range compatible with various methods of staining, such as SYPRO® Ruby Protein Gel Stain or Silver Staining?
  5. I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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