BioXp® error-corrected libraries kits

BioXp error-corrected libraries kits are built using proprietary technology that leverages de novo DNA synthesis, error correction, and amplification. The final product is purified library gene fragments ready for downstream applications such as cloning, screening, or selection.

Through the power of push-button, end-to-end automation of the BioXp system, error-corrected libraries are assembled in a single instrument run of less than 16 hours – regardless of design complexities – enabling researchers to significantly reduce synthesis bottlenecks during Design-Build-Test cycles of lead optimization.

Available on both the BioXp 3250 and 9600 platforms.

 

Accelerate your path to discovery

Library synthesis time is independent of design complexity

Confidence in your results

DNA with the diversity you need & the fidelity you expect

Solve complex challenges

Escape from the design limitations of service providers

 

BioXp error-correction technology

Delivering both fidelity and diversity – from an overnight instrument run

 

The impact of error-correction technology on mean error rate of library builds on the BioXp

Sequence fidelity increases approximately 2-fold with error correction than without. Median error rate as measured across a pannel of representative standard libraries spanning 300-800bp, GC 30-60%

Diversity generation analysis of degenerate codons

Generation of diversity is unaffected by error-correction technology. Expected vs. observed amino acid distribution analysis from 32 unique NNK degenerate codons with and without error-correction.

Scanning libraries

BioXp scanning error-corrected libraries offer a wide variety of libraries with varied amino acid sites which can be categorized into simple-scanning (single amino acid/codon substitutions per well) and site-saturation libraries that employ NNN, NNK, and NNS degenerate codons.

Quickly identify AA sites that are sensitive to effect

Product specifications

Product format: Linear dsDNA

Median error rate: 1 in 5,000 bp

Size range: 300-800 bp*

GC content: 30-60%**

Degenerate codons: NNN, NNK, NNS

Max # degenerate codons: 3 contiguous, 6 non-contiguous per sequence

Configuration: 8, 16, 24, 32, 48, 72, or 96 synthesis reactions

Yield: >200 ng per library construct

System runtime: Up to 16.5 hrs

Classification: Research use only

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