Principle

Clean-up of PCR products in flexible 8-well strip format and for high throughput in convenient 96-well plate format. Formerly known as NucleoSpin 8 / 96 Extract II.

The NucleoSpin 8/96 PCR Clean-up kits combine an improved MACHEREY-NAGEL buffer chemistry with the design of the 8-well strips and 96-well plates and allow the direct purification of PCR products using vacuum manifolds and benchtop centrifuges.

The kits allow the rapid clean-up of up to 15 μg of DNA (100 bp–10 kbp) per preparation with high DNA recovery and reproducible yields. After purification with NucleoSpin 8/96 PCR Clean-up the highly pure PCR products are ready for immediate use in many applications, such as radioactive and fluorescent dye sequencing, including capillary sequencing, restriction analysis, ligation, and microarray analysis.

NucleoSpin PCR Clean-up Binding Strips/Plates can be used under vacuum or by centrifugation.
The NucleoSpin 8/96 PCR Clean-up kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the NucleoSpin 8/96 PCR Clean-up silica membrane. PCR products bind to the silica membrane under optimized conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5 mM Tris/HCl pH 8.5) or water (pH 8.0–8.5) and are ready-to-use directly for subsequent applications.
 

Required hardware

Centrifugation
For centrifuge processing of NucleoSpin 8 / 96 PCR Clean-up kits a microtiterplate centrifuge is required which is able to accommodate the NucleoSpin PCR Clean-up Strips/Plate stacked on a Round- or Square-well Block or Rack of Tube Strips and reaches accelerations of 5,600–6,000 x g (bucket height: 85 mm). For further information on suitable centrifuges please contact our Technical Service.
For centrifuge processing of the 8-well Strips the reusable Starter Set C, containing Column Holders C, NucleoSpin Dummy Strips, MN Square-well Blocks, Rack of Tube Strips is required.

Vacuum processing
The NucleoSpin 8 / 96 PCR Clean-up kits can be used manually with the NucleoVac 96 vacuum manifold.
For vacuum processing of the 8-well Strips the reusable Starter Set A, containing Column Holders A and NucleoSpin Dummy Strips is required. For automation on laboratory platforms with standard 96-well plate vacuum chambers the use of the Starter Set A is also required.

Features