PstI

Name: PstI
Brand: New England Biolabs
Price: 329.000 EUR
Availability: InStock

Product information

Code Name Size Quantity Price

R0140S

PstI, recombinant

10.000 units ( 20000 units/ml )

Unavailable in your region

R0140T

PstI, recombinant, conc.

10.000 units ( 100000 units/ml )

Unavailable in your region

R0140L

PstI, recombinant

50.000 units ( 20000 units/ml )

Unavailable in your region

R0140M

PstI, recombinant, conc.

50.000 units ( 100000 units/ml )

Unavailable in your region

R0140S

PstI, recombinant

10.000 units

- 1 +

Unavailable in your region

R0140T

PstI, recombinant, conc.

10.000 units

- 1 +

Unavailable in your region

R0140L

PstI, recombinant

50.000 units

- 1 +

Unavailable in your region

R0140M

PstI, recombinant, conc.

50.000 units

- 1 +

Unavailable in your region

Home Restriction Endonucleases PstI

PstI

PstI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10237495. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence C_TGCA^G

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3140) supplied with rCutSmart^™ Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: CTGCA/G

Catalog #	Concentration	Size
R0140S	20,000 units/ml	10,000 units
R0140T	100,000 units/ml	10,000 units
R0140L	20,000 units/ml	50,000 units
R0140M	100,000 units/ml	50,000 units
Please enter a quantity for at least one size

Product Information

PstI has a High Fidelity version PstI-HF^® (NEB #R3140).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).

This product is related to the following categories:: Restriction Endonucleases P R,; Time-Saver Qualified Restriction Enzymes

This product can be used in the following applications:: BioBrick® Assembly,; Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0140S

-20

PstI	R0140SVIAL	-20	1 x 0.5 ml	20,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0140T

-20

PstI	R0140TVIAL	-20	1 x 0.1 ml	100,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0140L

-20

PstI	R0140LVIAL	-20	2 x 1.25 ml	20,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	2 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0140M

-20

PstI	R0140MVIAL	-20	1 x 0.5 ml	100,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of PstI required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 75%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 50%

Diluent Compatibility
- Diluent C
Storage Buffer

250 mM NaCl
10 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

PstI-HF
Related Products
Companion Products
- PstI-HF®
Materials Sold Separately
- Gel Loading Dye, Purple (6X)
Product Notes
1. May exhibit star activity in rCutSmart Buffer.
2. Not sensitive to CpG, dcm, or dam methylation.

Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Digest Protocol
  
  Double Digest Protocol with Standard Restriction Enzymes
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Heat Inactivation
  
  NEBuffer Activity/Performance Chart with Restriction Enzymes
  
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Star Activity
  
  Traditional Cloning Quick Guide
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Sequences
  
  Cleavage of Supercoiled DNA
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoelectric Points (pI) for Restriction Enzymes
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Time-Saver™ Qualified Enzymes
  
  Why Choose Recombinant Enzymes?
- Web Tools
  Competitor Cross-Reference Tool
  
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter™ v3.0
  
  NEBioCalculator®
  
  REBASE®
FAQs & Troubleshooting
- FAQs
  What is the activity of PstI at 25°C?
  
  Is PstI used in special techniques?
  
  When is star activity a problem for PstI?
  
  What is the molecular weight of PstI?
  
  Is PstI blocked by methylation?
  
  Is PstI inhibited by dUTP incorporated at the site?
  
  Does spermidine increase activity?
  
  Are there specific surrounding sequences that produce slow or resistant sites?
  
  Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  
  Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  
  Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
- Troubleshooting
  Restriction Enzyme Troubleshooting Guide
- Tech Tips
  
  Blocked by some types of methylation often found in plant DNA.
Citations & Technical Literature
- Citations
  Product Citation Tool
  
  Additional Citations
  
  Yang GQ, Chen YM, Wang JP, Guo C, Zhao L, Wang XY, Guo Y, Li L, Li DZ, Guo ZH. (2016) Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants. Plant Methods; Aug 4;12, 39.
  
  Guo B, DeFaveri J, Sotelo G, Nair A, Merilä J. (2015) Population genomic evidence for adaptive differentiation in Baltic Sea three-spined sticklebacks. BMC Biol; Mar 24;13:19,
  
  Combosch DJ, Vollmer SV. (2015) Trans-Pacific RAD-Seq population genomics confirms introgressive hybridization in Eastern Pacific Pocillopora corals. Mol Phylogenet Evol; Jul;88, 154-62.
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  PstI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10237495. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
  
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0140S_L_v2
  
  R0140T_M_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0140S_L_v1_0411402
  
  R0140S_L_v1_0411302
  
  R0140S_L_v1_0411308
  
  R0140S_L_v1_0411408
  
  R0140T_M_v1_0411302
  
  R0140T_M_v1_0411310
  
  R0140T_M_v1_0411402
  
  R0140T_M_v1_0411409
  
  R0140S_L_v1_0411411
  
  R0140S_L_v1_0411505
  
  R0140T_M_v1_0411506
  
  R0140T_M_v1_0411510
  
  R0140S_L_v1_0411510
  
  R0140T_M_v1_0411603
  
  R0140S_L_v1_0411604
  
  R0140T_M_v1_0421608
  
  R0140S_L_v1_0421610
  
  R0140T_M_v1_0421701
  
  R0140T_M_v1_0421703
  
  R0140S_L_v1_0421701
  
  R0140S_L_v1_0421706
  
  R0140T_M_v1_0421707
  
  R0140S_L_v1_0421712
  
  R0140T_M_v1_0421712
  
  R0140S_v1_10015087
  
  R0140L_v1_10015092
  
  R0140M_v1_10030435
  
  R0140S_v1_10028864
  
  R0140T_v1_10030438
  
  R0140M_v1_10036321
  
  R0140T_v1_10036323
  
  R0140S_v1_10048617
  
  R0140T_v1_10047843
  
  R0140M_v1_10050298
  
  R0140L_v1_10051128
  
  R0140S_v1_10051126
  
  R0140M_v1_10056490
  
  R0140T_v1_10056488
  
  R0140S_v1_10057188
  
  R0140S_v1_10063188
  
  R0140L_v1_10064801
  
  R0140S_v1_10070419
  
  R0140L_v1_10078806
  
  R0140M_v1_10086924
  
  R0140S_v1_10084583
  
  R0140L_v1_10091776
  
  R0140M_v1_10091791
  
  R0140L_v1_10096986
  
  R0140T_v1_10090783
  
  R0140M_v1_10098713
  
  R0140S_v1_10100990
  
  R0140S_v1_10114562
  
  R0140T_v1_10118606
  
  R0140L_v1_10126465
  
  R0140M_v1_10126463
  
  R0140L_v1_10134217
  
  R0140S_v1_10134292
  
  R0140T_v1_10140865
  
  R0140L_v1_10149536
  
  R0140T_v1_10151001
  
  R0140M_v1_10152536
  
  R0140S_v1_10155030
  
  R0140T_v1_10157913
  
  R0140L_v1_10171934
  
  R0140S_v1_10171936
  
  R0140M_v1_10183111
  
  R0140T_v1_10183113
  
  R0140M_v1_10200288
  
  R0140S_v1_10205225
  
  R0140L_v1_10205228
  
  R0140T_v1_10226033
  
  R0140M_v2_10237507
  
  R0140T_v2_10237515
  
  R0140L_v2_10237508
  
  R0140S_v2_10237506
  
  R0140L_v2_10274363
  
  R0140M_v2_10276640
  
  R0140S_v2_10273346
  
  R0140T_v2_10284002
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  PstI
  
  NEBuffer™ r3.1
  
  Gel Loading Dye, Purple (6X)
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.