Product Class: Other

M13KO7 Helper Phage

Product Introduction

M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
Catalog # Size Concentration
N0315S 1.8 ml 1 x 1011 pfu/ml

Product Information

Description

M13KO7 is an M13 derivative, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication (1). M13KO7 is able to replicate in the absence of phagemid DNA. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid is packaged preferentially and secreted into the culture medium.This allows easy production of single-stranded phagemid DNA for mutagenesis or sequencing.

Product Source

13KO7 Phage supernatant is isolated from infected E. coli ER2738 by a standard procedure.
This product is related to the following categories:
Other Products,
Phage Display Products,
cDNA Synthesis & Reverse Transcriptases Products,
This product can be used in the following applications:
Phage Display,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Lac Repressor on Plasmid

0

Affinity Tag

Other

Supplied As

Liquid

Sequence Files

Fasta GenBank

Product Notes

  1. NEB does not recommend the use of M13KO7 as a cloning vector. For cloning peptides displayed on M13 phage, we recommend the Ph.D.™ Peptide Display Cloning System.

References

  1. Vieira, J. and Messing, J. (1987). R. Wu and L. Grossman(Ed.), Methods Enzymol.. 153, 3-11. San Diego: Academic Press.
  2. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A laboratory Manual, (3rd ed.),. 3.17-3.32. Cold Spring Harbor: Cold Spring Harbor Laboratory Press..

Protocols, Manuals & Usage

Protocols

  1. Use of M13KO7 Helper Phage for isolation of single-stranded phagemid DNA

Tools & Resources

Web Tools

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.