Principle

Isolation of total DNA from diverse soil types

NucleoSpin® Soil for isolation of  total DNA from soil includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important as soil is very heterogenous in pH and the amounts of organic or anorganic matter like humic substances or polysaccharides. The silica membrane in NucleoSpin® Soil Columns is optimized for quantitative DNA binding. NucleoSpin® Bead Tubes, containing strong ceramic beads, allow a most efficient lysis of microorganisms in the sample. NucleoSpin® Inhibitor Removal Columns are included for convenient elemination of contaminants.
NucleoSpin® Soil allows processing of up to 500 mg (wet weight) starting material. Depending on the individual sample, typical yields are in the range of 2 to 10 μg DNA. The eluted DNA is ready-to-use (undiluted) for subsequent reactions like PCR, restriction analysis, and others.

Features