NucleoSpin® eDNA Water

Setting new standards for environmental DNA purification from water samples

The NucleoSpin® eDNA Water kit enables fast purification of environmental DNA (eDNA) from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix with silica membrane columns enables efficient isolation of high quality DNA. NucleoSpin® eDNA Water provides a workflow for eDNA extraction from filters in less than 70 minutes, avoiding long, over-night incubation steps typical of eDNA workflows. Columns are ethylene oxide treated in order to minimize the risk of DNA contamination. NucleoSpin® eDNA is compatible with a variety of filters and filtration systems (not included in the kit), such as conventional bottle top round filters (e.g. 45 mm) as well as cartridge filters (e.g. Sterivex™). Further, an alternative protocol for direct eDNA precipitation circumventing water filtration is provided, enabling isolation of free DNA. These properties make NucleoSpin® eDNA Water ideal for eDNA studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter.

Technical specification

Purification workflow

From filter to eDNA in under 70 minutes

The NucleoSpin® eDNA Water workflow has been optimized for speed and simplicity without compromizing the quality and purity of the DNA purified. Samples can be obtained either by filtration with round filters – like MACHEREY-NAGEL´s 45 mm Glass Fiber Filters (see ordering information) – or filter cartridges (Sterivex™). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from < 40 mL of unfiltered water (protocol included; requires additional buffer PREC). NucleoTrap ® Bead suspension efficiently binds the DNA, while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transfered to a NucleoSpin® XS column, from which pure eDNA is eluted. The purification workflow takes less than 70 min. The full preparation time depends on the sampling and / or filtration setup.

Options for sample pre-processing

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