Features

Isolation of microbial DNA, RNA, or both from diverse water and air samples

• Suitable for diverse salty and fresh water samples, ranging from turbid to clear as well as with air filters
• Minimized inhibition for reliable results
• Compatible with a variety of filters and filtration systems
• Can be additionally combined with two different sizes of ceramic bead tubes for optimal sample lysis

Technology	Magnetic bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Sample material	Water and air samples
Fragment size	300 bp–approx. 50 kbp
Elution volume	50–200 µL
Preparation time	40 min for KingFisher® Flex (excl. filtration and sample lysis)
Theoretical binding capacity	0.4 µg/µL beads

 

Applications*

• Microbial DNA and RNA from filtered water and air samples
• Manual and automated procedures at room temperature without reducing agents
• Typical downstream applications: qPCR, qRT-PCR, NGS

*Kits to be used for research purposes only!

 

Principle / Procedure

NucleoMag® DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear. NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including 25 mm and 47 mm filters from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality through determination of the microbial load of the sample. As DNA is extracted from the samples, presence of the entire microbiome, including coliform, pathogenic, as well as free living water microorganisms can be measured directly. The concurrent isolation of DNA and RNA allows for determination of the amount of living microorganisms in the sample and estimating, e.g., levels of gene expression as well as efficiencies of sterilization procedures. NucleoMag® DNA/RNA Water comes with magnetic beads and buffers included. The kit can additionally be combined with RNase, DNase, and two sizes of NucleoSpin® Bead Tubes Type A, providing a range of different options for the choice of filter and nucleic acid. In addition to manual nucleic acid purification, NucleoMag® DNA/RNA Water protocols are automation friendly and compatible with automation platforms suitable for magnetic bead purifications, such as KingFisher® and epMotion®. The kit does not require the addition of any reducing agents.

NucleoMag® DNA/RNA Water procedure

Application data

Efficient detection for different water and air samples
Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag® DNA/RNA Water kit.

Compatibility with different homogenization devices
A lysis reaction was performed with homogenization on either a Vortex-Genie® 2 device or a dedicated homogenization device. Both approaches delivered comparable results indicating the flexibility of the NucleoMag®DNA/RNA Water kit with regards of homogenization platforms.

Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different Filtration systems.

No PCR inhibition
Serial dilutions were performed for three different water samples. The linearity of the inhibition plots indicates the absence of PCR inhibition.

Automated protocol
In addition to manual processing, NucleoMag® DNA/RNA Water allows for automated workflows. Here, two different water samples were processed on two different automated platforms- a liquid handler and a magnetic rod system. Both systems delivered comparable results, indicating the adaptability of the kit to both automation approaches.