Features

Simple, fast, and convenient clean up of huge RNA amounts

• Up to 35 mg crude RNA can be used as sample material
• Complete removal of RT-PCR inhibitors
• Time saving procedure without DNase digestion

Applications

RNA clean-up of:
• Pre-purified RNA (e.g. NucleoZol, Trizol)
• Reaction mixtures
• DNase reaction mixtures

Principle / Procedure

The NucleoSpin® RNA Clean-up Maxi kit is designed to clean up large amounts of RNA from either crude preparations or from enzyme reactions.
One of the most important aspects during the isolation of RNA is to prevent degradation of the RNA. With the NucleoSpin® RNA Clean-up Maxi kit, RNA containing samples are mixed with a solution containing large amounts of chaotropic ions and ethanol. In this mixture, RNases are immediately inactivated and creates appropriate binding conditions to allow adsorption of RNA to the silica membrane. Three washing steps with two different wash buffers remove impurities. Pure RNA is finally eluted at low ionic strenght conditions with RNase-free water (supplied).The RNA clean up using NucleoSpin® RNA Clean-up Maxi kits can be performed at room temperature. The eluate should be treated with care because RNA is very sensitive to trace contaminations of RNases, often found on general lab ware, fingerprints and dust. Keep RNA frozen at -20 °C for short-term or -70 °C for longterm storage to ensure RNA stability.

Application data

NucleoSpin® RNA Clean-up Maxi is the perfect choice for clean-up of crude RNA sample up to 35 mg with excellent recovery
Three different crude RNA eluates have been used as input for further RNA clean-up using NucleoSpin® RNA Clean-up Maxi. The input amount of these crude RNA extracts varies from 35.4 mg to 6.8 mg. After RNA clean up according to the standard protocol, 30.1 mg to 6.6 mg of clean RNA was recovered. That RNA amount is equivalent to a recovery rate from 85 % to 96 %.

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