Principle

The NucleoSpin® RNA Plus XS kit is designed to purify RNA from small amounts of a variety of cell and tissue types. This kit supplies the NucleoSpin® gDNA Removal Column XS, a spin column that quickly and effectively removes genomic DNA without the need of DNase digestion.

One of the most important aspects during the isolation of RNA is to prevent degradation of the RNA. Cells and tissues are first lysed by incubation in a chaotropic ion Lysis Buffer (LB1 + LB2), which immediately inactivates RNases. The lysate is added to the NucleoSpin® gDNA Removal Column XS (yellow rings) to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution BSXS to the flow-through, the RNA is bound to the NucleoSpin® RNA Plus XS Column (light blue rings). Subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H2O.

The RNA preparation using NucleoSpin® RNA Plus XS kits can be performed at room temperature. The eluate should be treated with care because RNA is very sensitive to trace contaminations of RNases, often found on general lab ware, fingerprints, and dust. Keep RNA frozen at–20 °C for short-term or –70 °C for long-term storage to ensure RNA stability.

Features