Principle

For rapid medium and large scale preparation of genomic DNA from plant cells and fungi

The NucleoSpin® Plant II Midi / Maxi kit is designed for the isolation of genomic DNA from plant material and fungi. NucleoSpin® Plant II Midi allows processing of up to 400 mg starting material and NucleoSpin® Plant II Maxi up to 1500 mg. Depending on the individual sample, typical yields are in the range from 10 up to 100 μg DNA (Midi) and 50 up to 300 µg (Maxi). The eluted DNA is ready to use for Southern blotting, PCR, restriction analysis, and others.

Features

	NucleoSpin® Plant II Midi	NucleoSpin® Plant II Maxi
Technology	Silica-membrane technology
Format	Midi spin columns	Maxi spin columns
Sample material	<400 mg plant tissue (wet weight) <80 mg plant tissue (dry weight)	<1500 mg plant tissue (wet weight) <300 mg plant tissue (dry weight)
Lysate clarification	NucleoSpin® Filters L	NucleoSpin® Filters XL
Fragment size	50 bp–approx. 50 kbp	50 bp–approx. 50 kbp
Typical yield	10–100 µg	50–300 µg
A260/A280	1.8–1.9	1.8–1.9
Elution volume	200–400 µl	1000–3000 µl
Preparation time	90 min/prep	90 min/prep
Binding capacity	200 µg	500 µg