NucleoSpin® Blood L is designed for the rapid, medium scale preparation of highly pure genomic DNA from up to 2 mL whole blood, serum, plasma, or other body fluids. Buffy coat up to 1 mL can be processed easily. If enrichment of leukocytes is not possible, for example after freezing of blood which leads to lysis of cells, large volumes of blood samples have to be processed. The obtained DNA can be used directly as template for PCR reactions or can be subjected to endonuclease digestion.

Due to the high binding capacity of the NucleoSpin® Blood L Columns, which is about 250 μg of genomic DNA, the kit allows preparation of pure genomic DNA from up to 2 mL of human or animal whole blood with an A260/A280 ratio between 1.70 and 1.90. Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20–30 μg per ml of healthy human blood. Blood treated either with EDTA, citrate, heparin, or CPDA can be used. The procedure is optimized for a sample volume of 0.5–2 mL. Time for preparation of genomic DNA from blood with NucleoSpin® Blood L is less than 1 hour.

Nucleospin® Blood XL is designed for the rapid, large scale preparation of highly pure genomic DNA from up to 10 mL whole blood, serum, plasma, or other body fluids. Buffy coat volumes up to 2 mL can be processed easily. The obtained DNA can be used directly as template for PCR reactions or can be subjected to endonuclease digestion.

The binding capacity of the NucleoSpin® Blood XL columns is about 700 μg of genomic DNA. The kit allows preparation of pure genomic DNA from 2–10 mL of human whole blood with an A260/A280 ratio between 1.7 and 1.9. Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20–30 μg per ml of healthy human blood. Blood treated either with EDTA, citrate, heparin, or CPDA can be used. Time for preparation of genomic DNA from blood with NucleoSpin Blood XL is approximately 1 hour.

	NucleoSpin® Blood L	NucleoSpin® Blood XL
Technology	Silica-membrane technology
Format	Midi spin columns	Maxi spin columns
Sample material	0.2–2 mL whole blood 2 x 107 cultured cells	2–10 mL whole blood 108 cultured cells
Fragment size	200 bp–approx. 50 kbp	200 bp–approx. 50 kbp
Typical yield	40–60 µg	200–300 µg
A260/A280	1.6–1.9	1.6–1.9
Typical concentration	200–500 ng/µl	200–500 ng/µl
Elution volume	120–200 µl	600–1000 µl
Preparation time	~60 min/prep	~60 min/prep
Binding capacity	250 µg	700 µg