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Principle

For rapid small-scale isolation of genomic DNA from food and feed

Identification of GMO-DNA or animal components in food and feed has become an important application of public interest. In the novel food regulation, labelling has been mandatory for GMO containing food since they can be distinguished from conventional products by analytical methods, for example real-time PCR. Food samples are very heterogeneous and contain many different compounds like fat, cocoa or polysaccharides which can lead to suboptimal extraction or subsequent processing of DNA. NucleoSpin® Food guarantees good recovery rates even for small genomic DNA fragments (< 1 kb) out of processed, complex food matrices (e.g., ketchup or spices), which generally have very low DNA contents as well as poor quality, degraded DNA.

After the food samples have been homogenized, the DNA can be extracted with lysis buffers containing chaotropic salts, denaturing agents and detergents. The standard isolation ensures lysis using Buffer CF, which was especially developed in cooperation with GEN-IAL, Troisdorf, Germany (patent pending). Lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. The clear supernatant is then mixed with binding buffer and ethanol to create conditions for optimal binding to the NucleoSpin® silica membrane, which was selected for this purpose due to its unique DNA-binding properties. After washing with two different buffers for efficient removal of potential PCR inhibitors, DNA can be eluted in low salt buffer or water, and is ready-to-use in subsequent reactions.

Features

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