NucleoSpin® DNA Trace is designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell and forensic sample. The NucleoSpin® Funnel Columns (NucleoSpin® DNA Trace F Columns) included in the kit are ideally suited for collecting traces of nucleic acids from large volumes. The optimized shape of the NucleoSpin® Funnel Column allows the combination of large volume capacity and small diameter of the binding membrane. The DNA isolated by NucleoSpin® DNA Trace is ready-to-use for common downstream applications like PCR, real-time PCR, or other enzymatic reactions. MACHEREY-NAGEL as a manufacturer of silica and glass fibre filters was able to develop an optimized membrane for the reversible binding of very small quantities of nucleic acids from dilute samples. DNA amounts in the nanogram range can be processed with high recovery (e.g., blood traces: DNA from a sample as small as 0.2 μL could be isolated and amplified using LightCycler™ PCR, data not shown). On the other hand the silica membrane can bind up to 25 μg of genomic DNA.

The small diameter of the silica membrane allows elution volumes as low as 50 to 100 μL of low-salt buffer or water leading to highly enriched samples. 8 to 12 NucleoSpin® Funnel Columns can be processed in parallel depending on the centrifuge rotor used. The risk of cross contamination is eliminated by placing the funnel columns into 50 mL polyethylene tubes which can be closed with screw caps, resulting in self-contained systems. For elution, 0.5 mL elution tubes are fitted directly to the outlet of the funnel columns (collection tubes and elution tubes provided with the kit). For isolation of genomic DNA from human bones additional buffers are necessary. For this MACHEREY-NAGEL offers the customized NucleoSpin® DNA Trace Bone Buffer Set.

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