Principle

The second generation for rapid isolation of genomic DNA from plant samples with higher yield and quality

With NucleoSpin® Plant II MACHEREY-NAGEL introduces the second generation of kits for isolation of gDNA from plant material, fungi, and other biological samples. The kit includes two optimized, alternative lysis buffers based on the established CTAB and SDS lysis methods. As plants are very heterogenous and contain a lot of different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin® Plant II offers two different lysis procedures for optimal processing of various samples. The silica membrane in the NucleoSpin® Plant II Columns is optimized to improve DNA binding. NucleoSpin® Filters are included for convenient clarification of lysates. NucleoSpin® Plant II allows processing of up to 100 mg (wet weight) or 20 mg (dry weight) starting material. Depending on the individual sample typical yields are in the range from 1 to 30 μg DNA. The eluted DNA is ready to use for subsequent reactions like PCR, restriction analysis, and others.

Features