Principle

Time-saving isolation of genomic DNA from human or animal tissue, cultured cells, or bacteria for varying throughput in flexible 8-well strip format and for high throughput in approved 96-well format

NucleoSpin 8/96 Tissue is designed for the rapid simultaneous preparation of highly pure genomic DNA from animal and human tissue, e.g. mouse and rat tails. From a 1.2 cm mouse tail tip section (age of mice 4 – 6 weeks) up to 30 – 40 μg of pure genomic DNA can be prepared (typical yields 15 – 25 μg). The obtained DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction. NucleoSpin Tissue Binding Strips/Plates can be used under vacuum or by centrifugation. With the NucleoSpin 8/96 Tissue method, lysis is achieved by incubation of the samples in a solution containing detergents and proteinase K at 56 °C. Appropriate conditions for binding of DNA to the silica membranes of the NucleoSpin Tissue Binding Strip/Plates are created by addition of large amounts of chaotropic ions and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by washing with two different wash buffers. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer. The obtained DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

Features