Principle / Procedure

NucleoSpin® DNA Stool is a new kit for the purification of genomic DNA from bacterial and epithelial cells from stool samples. The isolated DNA is used for analysis of the microbiome or genotyping of the donor.

The NucleoSpin® DNA Stool kit utilizes NucleoSpin® Bead Tubes Type A (ceramic beads; included in kit) in combination with a common disruption device, e.g., MN Bead Tube Holder, or a swing mill to lyse different cell types and tissues within a single procedure. Alternative NucleoSpin® Bead Tubes can be ordered separately if needed. NucleoSpin® Inhibitor Removal Columns are included for convenient elimination of contaminants.

NucleoSpin DNA Stool procedure

Approx. 200 mg (180–220 mg)* stool samples (fresh or frozen)

Applications

• Genomic DNA from bacterial and epithelial cells from stool samples
• Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology