Product Class: Other

Exonuclease VIII, truncated
NEB4 cloned at NEB recombinant 37 70 Heat

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

Product Introduction

  • Double-stranded DNA exonuclease that preferentially degrades linear dsDNA
  • Initiates at the 5' termini of linear double-stranded DNA
  • Catalyzes the removal of nucleotides from linear double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease VIII, truncated can be used for:

  • Removal of linear double-stranded DNA, leaving behind circular DNA in the sample
Catalog # Size Concentration
M0545S 1000.0 units 10000 units/ml
M0545L 5000.0 units 10000 units/ml

Product Information

Description

Product Source

An E. coli strain that carries a plasmid with genetic engineering active domain of Exonuclease VIII.
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H] DNA.

Reaction Conditions

1X NEBuffer™ 4
Incubate at 37°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

50 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
0.1% Triton® X-100
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

70°C for 15 minutes

Unit Assay Conditions

1X NEBuffer 4 with 0.15 mM with sonicated duplex 3H- DNA.

Application Features

•    Degradation of linear dsDNA while maintaining double stranded circular DNA.

References

  1. Chang, et al. (2001). J. Biol. Chem.. 46004–46010.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Exonuclease VIII, truncated (M0545)

Tools & Resources

Selection Charts

Web Tools

Research Posters

FAQs & Troubleshooting

FAQs

  1. How does Exonuclease VIII, truncated differ from Lambda Exonuclease (NEB# M0262)?

  2. Will Exonuclease VIII, truncated cleave supercoiled dsDNA or covalently close circular form dsDNA?

  3. How does Exonuclease VIII, truncated differ from Exonuclease III (NEB# M0206)?
  4. Can DNA be blunted using Exonuclease VIII, truncated?
  5. What is the activity of Exonuclease VIII, truncated in these common buffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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