Principle

Kit for purification of high-quality DNA from gram-positive and gram-negative bacteria, yeast, and fungi

NucleoSpin® Microbial DNA is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi).
The NucleoSpin® Microbial DNA kit utilizes NucleoSpin® Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device e.g., swing mill, in order to lyse different sample materials with one procedure. The procedure is fast, simple and reliably high DNA yields from a wide range of microbial samples can be obtained. Alternative NucleoSpin® Bead Tubes can be ordered separately.
Appropriate DNA binding conditions to the NucleoSpin® Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer MG) to the lysate. Contaminants are removed by two efficient washing steps. Afterwards, DNA is eluted with a slight alkaline buffer and is ready to use for subsequent reactions.

NucleoSpin® Microbial DNA procedure

Features

Applications*

• Total DNA from microbial cultures
• Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions

* Kits to be used for research purposes only