Principle

Desalting tools for speeding up the time-consuming precipitation step after anion-exchange plasmid purification

The NucleoBond® Finalizers are designed for quick concentration and desalination of plasmid and cosmid DNA eluates that are obtained by anion-exchange chromatographic DNA purification with NucleoBond® columns. The sample is precipitated with isopropanol and loaded onto a NucleoBond® Finalizer membrane by means of a syringe. After an ethanolic washing step and drying of the membrane the pure DNA can be eluted with low salt buffer for further use. The NucleoBond® Finalizer technology replaces the tedious and time-consuming centrifugation step which hitherto follows every isopropanol precipitation of diluted DNA samples. Furthermore problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented. The pure concentrated and almost salt free DNA can be used directly for all common down-stream applications like transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR.

Features