Principle

Isolation of genomic DNA from whole blood in flexible 8-well strip format and for high throughput in convenient 96-well format

NucleoSpin® 8 / 96 Blood QuickPure is designed for the rapid simultaneous preparation of genomic DNA from whole blood, serum, plasma and body fluids (amniotic fluid, liquor etc.). Animal or human blood samples, fresh or frozen material, may be processed conveniently. NucleoSpin® Blood QuickPure Binding Strips/Plates can be used by centrifugation. The method is based on a specially treated silica membrane which allows to speed up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to only 1! Therefore, the hands-on-time is minimized. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of Proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin® Blood QuickPure Binding Strips/Plates are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR, Southern blotting, or any kind of enzymatic reactions.

Features

 * For preparation of 300 µL samples increased volumes of Lysis Buffer BQ1 required.