Product Class: Other

Monarch® Plasmid Miniprep Columns 

This product will be discontinued by December 2024 or sooner, while supplies last.

A new version of these columns and tubes are now available, featuring upgraded spin columns. Migrate to the new Monarch Spin Columns S2D and Tubes (NEB #T1117).

Product Introduction

  • A component of the Monarch Plasmid Miniprep Kit (NEB #T1010)
  • Includes 100 spin columns and 100 collection tubes
  • Binding capacity of 20 µg 

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Catalog # Size Concentration
T1017L 100.0 columns

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Product Information

Description

Highlights


  • No buffer retention/risk of carryover contamination
  • Low elution volume (≥ 30 μl)
  • Convenient tab for easy labeling
  • Snug fit into collection tubes for easy handling
  • Made with less plastic than conventional columns

Monarch Plasmid Miniprep Columns, supplied with the Monarch Plasmid Miniprep Kit, have been custom designed to deliver excellent performance for your plasmid purification. Monarch Columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carry-over contamination. The tapered design of the Miniprep Column enables elution in as little as 30 μl. Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. The columns fit snugly into the collection tubes to enable easy handling. The Monarch Miniprep Columns also feature a convenient tab so that you can label your columns easily without removing them from your tube rack. Monarch columns are suitable for use with centrifugation and vacuum purification protocols, and are supplied with collection tubes.


Monarch Plasmid Miniprep Column Design
This product is related to the following categories:
Plasmid Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Binding Capacity

20 µg

Protocols, Manuals & Usage

Protocols

  1. Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
  2. I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
  3. If I need to clean up more than 5 μg of DNA, is there another higher capacity column alternative to the columns included in the Monarch PCR & DNA Cleanup kit (NEB #T1030)?
  4. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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