Product Class: Other

Monarch® Plasmid Neutralization Buffer (B3)

This product will be discontinued by October 2024 or sooner, while supplies last.

Learn more about Monarch Nucleic Acid Purification Kits

Product Introduction

  • Monarch buffers and columns are all sold separately for your convenience. Keep in mind that this buffer contains RNase A and will need to be stored at 4°C after opening.
  • Monarch Plasmid Neutralization Buffer is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L).
  • This buffer is used to neutralize the lysate and digest any RNA present. The buffer also prepares the DNA for binding to the column matrix.
  • It is conveniently colored yellow for identification as well as for monitoring when the neutralization is complete.
Catalog # Size Concentration
T1013L 110.0 ml

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Product Information

Description

This product is related to the following categories:
Plasmid Purification Products,
Buffers Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Product Notes

  1. This buffer contains RNAse A and will need to be stored at 4°C after opening.

Protocols, Manuals & Usage

Protocols

  1. Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
  2. I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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