Product Class: Other

Monarch® Plasmid Lysis Buffer (B2)

This product will be discontinued by November 2024 or sooner, while supplies last.

An alternative version of this product can be found in the new Monarch Spin Plasmid Miniprep Kit (NEB #T1110).

Learn more about Monarch Nucleic Acid Purification Kits.

Product Introduction

  • A component of the Monarch Plasmid Miniprep Kit (NEB #T1010)
  • Used to lyse cells under alkaline conditions
  • Colored blue for monitoring lysis progress and for easy identification
Catalog # Size Concentration
T1012L 54.0 ml

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Product Information

Description

Monarch Plasmid Lysis Buffer (B2) is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). The Lysis buffer is used to break open the cells under alkaline conditions in order to release the cellular components, including the plasmid DNA. For easy identification, the buffer is colored blue. This also helps to monitor the completion of the cell lysis step.

This product is related to the following categories:
Plasmid Purification Products,
Buffers Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
  2. I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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