Product Class: Other

Chitin Resin

Product Introduction

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion

  • Strong specific binding for CBD-fusion protein affords highly pure protein of interest from crude lysate in one step
  • Removal of CBD-tag during elution typically yields highly pure, native protein without the use of a protease
  • This resin, when used with pTXB1, allows for the isolation of native recombinant proteins possessing a reactive C-terminal thioester that can be used for applications in intein-mediated protein ligation (IPL) and site-specific labeling
  • Binding capacity: 2 mg maltose-binding protein/mL bed volume released from the resin after cleavage of the maltose-binding-CBD-fusion protein
  • Resin may be regenerated up to 5 times
Catalog # Size Concentration
S6651S 20.0 ml
S6651L 100.0 ml

Product Information

Description

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion (1)

IMPACT Citations

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

This product is related to the following categories:
IMPACT System,
Bacterial E. coli Protein Expression Products,
Chitin Purification (CBD-tag),
Affinity Purification Products,
Protein Purification Products,
Protein Expression Products,
This product can be used in the following applications:
Affinity Purification & Expression Tags,
Protein Purification,
IMPACT Affinity Tag

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Support Matrix

Approximately 50-70 μm microparticles

Binding Capacity

2.0 mg maltose-binding protein/ ml bed volume released from the resin after cleavage of the fusion protein expressed from pMYB5.

Can be Regenerated

Yes

Product Notes

  1. We recommend using gravity flow to run thecolumn. The column should have a wide diameter.
  2. The column may be regenerated 5 times.
  3. Store at 4°C. The used resin should be stored incolumn buffer. For long term storage add 0.02%sodium azide to the column buffer.

References

  1. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene.

Protocols, Manuals & Usage

Protocols

  1. Affinity Purification and On-column Cleavage (NEB #S6651)

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What gravity column do you recommend?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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