BspQI
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0712S |
BspQI, recombinant |
500 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0712L |
BspQI, recombinant |
2.500 units ( 10000 units/ml ) | - | Unavailable in your region |
BspQI
Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments). BspQI has been reformulated, and now also includes Recombinant Albumin (rAlbumin), beginning with Lot #10201944. Learn more. GMP-grade reagent also available. Learn more. We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Product Introduction
- Linearize plasmid template before in vitro transcription
- Time-Saver™ qualified for digestion in 5-15 minutes
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Restriction Enzyme Cut Site: GCTCTTC(1/4)
- Used in Golden Gate Assembly
- Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly
Catalog # | Size | Concentration |
---|---|---|
R0712S | 500.0 units | 10000 units/ml |
R0712L | 2500.0 units | 10000 units/ml |
Featured Videos
View Video Library-
Golden Gate Assembly Workflow
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Golden Gate Assembly Domestication Tutorial
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Golden Gate Assembly Tool Tutorial
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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Restriction Enzymes in Golden Gate Assembly
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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Function and Application of Type IIS Restriction Enzymes
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Product Source
An E. coli strain that carries the cloned BspQI gene from Bacillus sphaericus (X.S. Pan)- This product is related to the following categories:
- Restriction Endonucleases B Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- High-throughput cloning and automation solutions,
- NEBridge® Golden Gate Assembly ,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µl.Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 50°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 100%NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Activity at Temperature
@37°C: 50%Related Products
Companion Products
Product Notes
- Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
- Star activity may result in a low salt buffer, such as rCutSmart Buffer. For an optimal restriction digest protocol please see NEBCloner.
- Not sensitive to CpG, dcm, or dam methylation.
- star activity may result from a glycerol concentration of >5%
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Cleavage of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0712S_L_v1_0021301
- R0712S_L_v1_0021303
- R0712S_L_v1_0021308
- R0712S_L_v1_0021401
- R0712S_L_v1_0021404
- R0712S_L_v1_0021407
- R0712S_L_v1_0021411
- R0712S_L_v1_0021501
- R0712S_L_v1_0021504
- R0712S_L_v1_0021409
- R0712S_L_v1_0021507
- R0712S_L_v1_0021509
- R0712S_L_v1_0021511
- R0712S_L_v2_0021602
- R0712S_L_v2_0021606
- R0712S_L_v1_0021602
- R0712S_L_v1_0021606
- R0712S_L_v2_0021701
- R0712S_L_v2_0021706
- R0712S_L_v2_0021712
- R0712S_v2_10014986
- R0712L_v2_10014984
- R0712S_v2_10025868
- R0712L_v2_10025864
- R0712L_v2_10037231
- R0712S_v2_10037233
- R0712L_v2_10052832
- R0712S_v2_10058174
- R0712S_v2_10064760
- R0712L_v2_10069701
- R0712S_v2_10076496
- R0712S_v2_10080038
- R0712L_v2_10086321
- R0712S_v2_10091199
- R0712S_v2_10093546
- R0712L_v2_10097373
- R0712L_v2_10100070
- R0712L_v2_10106387
- R0712S_v2_10108647
- R0712L_v2_10109213
- R0712S_v2_10111561
- R0712S_v2_10124014
- R0712L_v2_10131863
- R0712L_v2_10117176
- R0712L_v2_10138624
- R0712S_v2_10138626
- R0712L_v2_10144731
- R0712L_v2_10151405
- R0712S_v2_10155950
- R0712L_v2_10156274
- R0712L_v2_10162291
- R0712L_v2_10167272
- R0712S_v2_10167276
- R0712L_v2_10173042
- R0712L_v2_10178132
- R0712L_v2_10183989
- R0712L_v2_10185366
- R0712S_v2_10185370
- R0712L_v2_10193693
- R0712S_v2_10201883
- R0712L_v3_10203108
- R0712S_v3_10203109
- R0712L_v3_10207982
- R0712S_v3_10207985
- R0712L_v3_10221733
- R0712S_v3_10217770
- R0712S_v3_10233277
- R0712L_v3_10233543
- R0712L_v3_10241997
- R0712L_v3_10254243
- R0712S_v3_10254242
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.BspQI
NEBuffer™ r3.1
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.