NotI
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0189S |
NotI, recombinant |
500 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0189L |
NotI, recombinant |
2.500 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0189M |
NotI, recombinant, conc. |
2.500 units ( 50000 units/ml ) | - | Unavailable in your region |
NotI
NotI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10232248. Learn more.
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- High Fidelity (HF®) version available (NEB #R3189) supplied with rCutSmart™ Buffer
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Restriction Enzyme Cut Site: GC/GGCCGC
Catalog # | Size | Concentration |
---|---|---|
R0189S | 500.0 units | 10000 units/ml |
R0189L | 2500.0 units | 10000 units/ml |
R0189M | 2500.0 units | 50000 units/ml |
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- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
NotI has a High Fidelity version NotI-HF® (NEB #R3189).High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product Source
An E. coli strain that carries the cloned NotI gene from Nocardia otitidis-caviarum (ATCC 14630).- This product is related to the following categories:
- Restriction Endonucleases: N-O Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pBC4 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 37°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: <10%NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 25%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Related Products
Companion Products
Materials Sold Separately
Product Notes
- Blocked by CpG methylation.
References
- Samuelson, J.C., Morgan, R.D., Benner, J.S., Claus, T.E., Packard, S.L. and Xu, S.Y. (2006). Engineering a rare-cutting restriction enzyme: Genetic screening and selection of NotI variants. Nucleic Acids Research. 34, 796-805. PubMedID: 16456032
- Zhang, P., Bao, Y., Higgins, L. and Xu, S.Y. (2007). Rational design of a chimeric endonuclease targeted to NotI recognition site. Protein Engineering, Design and Selection. 20, 497-504.
- Lambert, A.R., Sussman, D., Shen, B., Maunus, R., Nix, J., Samuelson, J., Xu, S.Y. and Stoddard, B.L. (2008). Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding. Structure. 16, 558-569.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Time-Saver™ Qualified Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
NotI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10232248. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0189M_v1_0551310
- R0189M_v1_0551401
- R0189S_L_v1_0551309
- R0189S_L_v1_0551404
- R0189M_v1_0521212
- R0189M_v1_0521304
- R0189S_L_v1_0521212
- R0189S_L_v1_0521304
- R0189S_L_v1_0551409
- R0189S_L_v1_0551504
- R0189M_v1_0551504
- R0189M_v1_0551404
- R0189M_v1_0551509
- R0189S_L_v1_0551509
- R0189M_v1_0551602
- R0189S_L_v1_0551602
- R0189S_L_v1_0551609
- R0189M_v1_0551610
- R0189S_L_v1_0551703
- R0189M_v1_0551703
- R0189M_v1_0551708
- R0189S_L_v1_0551708
- R0189S_L_v1_0551712
- R0189M_v1_0551804
- R0189S_v1_10014910
- R0189S_v1_10014138
- R0189S_v1_10021238
- R0189L_v1_10014136
- R0189S_v1_10030465
- R0189L_v1_10030463
- R0189M_v1_10034205
- R0189S_v1_10034833
- R0189S_v1_10040261
- R0189S_v1_10044742
- R0189S_v1_10048637
- R0189L_v1_10048636
- R0189S_v1_10054488
- R0189M_v1_10055657
- R0189S_v1_10059971
- R0189S_v1_10063443
- R0189M_v1_10065830
- R0189S_v1_10068945
- R0189L_v1_10068947
- R0189S_v1_10076569
- R0189S_v1_10081427
- R0189L_v1_10084431
- R0189L_v1_10091142
- R0189S_v1_10087628
- R0189S_v1_10091122
- R0189M_v1_10094434
- R0189S_v1_10096377
- R0189S_v1_10103312
- R0189L_v1_10104223
- R0189S_v1_10110992
- R0189L_v1_10117802
- R0189S_v1_10117149
- R0189M_v1_10118763
- R0189S_v1_10126194
- R0189L_v1_10128900
- R0189S_v1_10136147
- R0189L_v1_10146679
- R0189S_v1_10141492
- R0189M_v1_10148594
- R0189S_v1_10152197
- R0189M_v1_10159209
- R0189S_v1_10161018
- R0189L_v1_10171835
- R0189S_v1_10171833
- R0189S_v1_10181577
- R0189S_v1_10185508
- R0189L_v1_10181579
- R0189M_v1_10191041
- R0189S_v1_10191040
- R0189S_v1_10204880
- R0189L_v1_10204881
- R0189L_v1_10211215
- R0189S_v1_10211217
- R0189L_v1_10215202
- R0189S_v1_10230049
- R0189S_v1_10229436
- R0189M_v2_10232268
- R0189L_v2_10232254
- R0189S_v2_10232253
- R0189S_v2_10249236
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.NotI
NEBuffer™ r3.1
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.