XmaI
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0180S |
XmaI, recombinant |
500 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0180L |
XmaI, recombinant |
2.500 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0180M |
XmaI, recombinant, conc. |
2.500 units ( 50000 units/ml ) | - | Unavailable in your region |
XmaI
XmaI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10180742. Learn more. We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Restriction Enzyme Cut Site: C/CCGGG
Catalog # | Size | Concentration |
---|---|---|
R0180S | 500.0 units | 10000 units/ml |
R0180L | 2500.0 units | 10000 units/ml |
R0180M | 2500.0 units | 50000 units/ml |
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- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
Product Source
An E. coli strain that carries the XmaI gene from Xanthomonas malvacearum (ATCC 9924).- This product is related to the following categories:
- Restriction Endonucleases T Z Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg pXba in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 25%NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Impaired
Isoschizomers
Related Products
Materials Sold Separately
Product Notes
- XmaI is an isoschizomer of SmaI. XmaI produces a 5´ extension whereas SmaI produces blunt-ended fragments.
- Impaired by CpG methylation.
- star activity may result from a glycerol concentration of >5%
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction enzymes requiring multi-sites for efficient cleavage
- Single Letter Codes
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Time-Saver™ Qualified Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Is XmaI activity sensitive to dam, dcm or mammalian CpG methylation?
- How many base pairs should be added at the end of a PCR primer after the XmaI recognition site to guarantee that XmaI will cut properly?
- Does XmaI have any isoschizomers/neoschizomers?
- Does XmaI exhibit star activity?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0180M_v1_0661308
- R0180S_L_v1_0661308
- R0180S_L_v1_0661401
- R0180M_v1_0651303
- R0180M_v1_0661401
- R0180M_v1_0661406
- R0180S_L_v1_0651303
- R0180S_L_v1_0661406
- R0180M_v1_0661411
- R0180S_L_v1_0661411
- R0180M_v1_0661504
- R0180S_L_v1_0661504
- R0180M_v1_0661510
- R0180S_L_v1_0661510
- R0180M_v1_0661512
- R0180S_L_v1_0661603
- R0180S_L_v1_0661611
- R0180M_v1_0661705
- R0180S_L_v1_0661710
- R0180M_v1_0661801
- R0180S_L_v1_0661804
- R0180L_v1_10010824
- R0180S_v1_10017832
- R0180M_v1_10021272
- R0180L_v1_10022526
- R0180S_v1_10022745
- R0180S_v1_10030769
- R0180S_v1_10035141
- R0180M_v1_10035139
- R0180L_v1_10035137
- R0180S_v1_10043451
- R0180M_v1_10049934
- R0180S_v1_10049607
- R0180M_v1_10052662
- R0180S_v1_10054574
- R0180L_v1_10054572
- R0180S_v1_10058307
- R0180S_v1_10064937
- R0180L_v1_10064612
- R0180M_v1_10069147
- R0180S_v1_10074027
- R0180L_v1_10077758
- R0180S_v1_10078783
- R0180S_v1_10081731
- R0180S_v1_10084405
- R0180L_v1_10086000
- R0180M_v1_10091242
- R0180S_v1_10091120
- R0180S_v1_10088355
- R0180S_v1_10096866
- R0180L_v1_10096864
- R0180S_v1_10101093
- R0180M_v1_10107187
- R0180S_v1_10107804
- R0180S_v1_10117551
- R0180S_v1_10121639
- R0180L_v1_10126482
- R0180S_v1_10134294
- R0180M_v1_10135732
- R0180S_v1_10140658
- R0180L_v1_10145344
- R0180S_v1_10147207
- R0180M_v1_10149515
- R0180L_v1_10155954
- R0180S_v1_10155956
- R0180S_v1_10164028
- R0180M_v1_10169567
- R0180L_v2_10180776
- R0180M_v2_10180775
- R0180S_v2_10180774
- R0180S_v2_10186593
- R0180S_v2_10191656
- R0180S_v2_10202625
- R0180M_v2_10204867
- R0180S_v2_10209272
- R0180S_v2_10225988
- R0180L_v2_10209270
- R0180S_v2_10234994
- R0180M_v2_10245741
- R0180S_v2_10253164
- R0180L_v2_10253163
- R0180S_v2_10269857
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.XmaI
rCutSmart™ Buffer
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.