BglII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10237497. Learn more.
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
10 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity
dam methylation: Not Sensitive dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping
Product Notes
The various sticky ends produced by BglI cleavage can be used to reconstitute plasmid and phage genomes and to exchange wild-type and mutant DNA fragments (Burger, K. J. and Schinzel, R. (1983) Mol. Gen. 189, 269-274).
This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.
Blocked by some combinations of overlapping CpG methylation.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
