Product Class: Other

Anti-SNAP-tag® Antibody (Polyclonal)

Product Introduction

The Anti-SNAP-tag® Antibody (Polyclonal) can be used for the detection of SNAP-tagged fusion proteins in Western blots.

  • This antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, and 50% glycerol
  • The sensitivity of detection is 5 ng of SNAP-tag per load in Western blotting
  • Recommended for Western blot applications only
Catalog # Size Concentration
P9310S 100.0 µl

Product Information

Description

This rabbit polycolonal antibody was obtained from the immunization with purified recombinant SNAP-tag® protein and affinity purified using SNAP-BG resin. 

Western blot analysis of SNAP-tag fusion proteins expressed in mammalian cellsWestern blot analysis of SNAP-tag fusion proteins expressed in mammalian cells.
CHO-K1 cells were transfected with plasmids expressing histone protein H2B or adenoviral protein E3-14.7K fused to SNAP-tag. The cell lysates, each prepared from 5x103 cells, were electrophoresed on a 10–20% Tris-glycine polyacrylamide gel under denaturing conditions. Western blot analysis was performed with anti-SNAP antibody in 1:1000 dilution and chemiluminescent detection. Control: untransfected cell lysate.
This product is related to the following categories:
Snap Tag Other Products,
Protein Labeling Products,
This product can be used in the following applications:
SNAP Companion,
Protein Labeling Snap Clip,
Cell Imaging

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Antibody Type

Polyclonal

Recommended Dilution for Western Blot

1:1000

Recommended Applications

Detection Method

Western Blot

Sensitivity

5 ng

Product Notes

  1. Storage: This antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, and 50% glycerol. Store undiluted at -20°C for up to two years. Avoid repeated elevation in temperature.
  2. The optimal dilution for Western blotting may have to be experimentally determined to account for protein expression and assay conditions.  The recommended starting dilution is 1:1000.
  3. The sensitivity of detection is 10 ng of SNAP-tag per load in Western blotting.
  4. This antibody has not been validated for other applications.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)

FAQs & Troubleshooting

FAQs

  1. Cellular Imaging and Analysis FAQs

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In