Product Class: Other

Enterokinase, light chain
recombinant

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Product Introduction

Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
Catalog # Size Concentration
P8070S 480.0 units 16000 units/ml
P8070L 2560.0 units 16000 units/ml

Product Information

Description

Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.

Product Source

This preparation is purified from Pichia pastoris containing a clone of the light chain of the bovine enterokinase gene.
This product is related to the following categories:
Proteases Products
This product can be used in the following applications:
Fusion Protein Cleavage,
Protein Purification,
Protein Digestion

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 µl.

Removal

Enterokinase will bind specifically to trypsin inhibitor agarose.

Storage Buffer

20 mM Tris-HCl
200 mM NaCl
2 mM CaCl2
50% Glycerol
pH 7.2 @ 4°C

Molecular Weights

Apparent: 31 kDa
Theoretical: 26.3 kDa

Unit Assay Conditions

Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE.

Product Notes

  1. NaCl concentration above 250 mM will significantly inhibit enzyme activity.
  2. Removal: Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).
  3. Enterokinase is a glycosylated protein.

Protocols, Manuals & Usage

Protocols

  1. Protocol for use with Enterokinase, light chain (P8070)

Tools & Resources

Selection Charts

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is sold under patent license from WYETH for RESEARCH USE ONLY. Licenses for commercial manufacture or use may be obtained directly from WYETH, 87 Cambridgepark Drive, Cambridge, MA 02140.

Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs, Inc. and DSM Biologics Company B.V. The buyer and user has a non-exclusive sub-license to use this system or any component thereof, including the K. lactis GG799 competent cells for RESEARCH PURPOSES ONLY. A license to use this component for manufacture or clinical grade material for commercial purposes is available from New England Biolabs, Inc. or DSM Biologics Company B.V.

Trademarks

NEW ENGLAND BIOLABS® is a registered trademark of New England Biolabs, Inc. 

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