α1-3,4 Fucosidase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
P0769S |
alpha 1-3,4 Fucosidase |
200 units ( 4000 units/ml ) | - | Unavailable in your region |
α1-3,4 Fucosidase
Product Introduction
α1-3,4 Fucosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-3 and α1-4 linked fucose residues from oligosaccharides and glycoproteins.
• Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
• Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
• ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
• Optimal activity and stability for up to 12 months
Catalog # | Size | Concentration |
---|---|---|
P0769S | 200.0 units | 4000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
Product Source
Cloned from the sweet almond tree (Prunus dulcis) and expressed in Pichia pastoris.Specificity
- This product is related to the following categories:
- Exoglycosidases Products,
- Proteome Analysis Products,
- This product can be used in the following applications:
- Expression Systems,
- Glycan Sequencing,
- Proteomics,
- Recombinant Glycoprotein Expression, Glycoprotein Analysis
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the α-fucose from 1 nmol of Galβ1-4GlcNAcβ1-3(Fucα1-3)Galβ1- 4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 μlReaction Conditions
1X GlycoBuffer 1
Supplement with 100 µg/ml Recombinant Albumin (Low-glycerol)
Incubate at 37°C
1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)
Storage Buffer
20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 @ 25°C
Heat Inactivation
65°C for 10 minutesMolecular Weight
Apparent: 56000 daltonsUnit Assay Conditions
Two fold dilutions of a1-3,4 Fucosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1 supplemented with 1X rHSA in a 10 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography (1).References
- Wong-Madden, S. T. and Landry, D. (1995). Glycobiology. 5, 19-28.
Protocols, Manuals & Usage
Protocols
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- What is the difference between α1-2 Fucosidase, α1-3,4,6 Fucosidase and α1-3,4 Fucosidase?
- Can I use α1-3,4 Fucosidase in a double digest with other exoglycosidases and/or endoglycosidases?
- Can α1-3,4 Fucosidase cleave core α1-3 fucose residues?
- Can α1-3,4 Fucosidase and α1-2,3,4,6 Fucosidase cleave branched and complex substrates?
- What are Glycosidases and their uses?
- Do detergents inhibit glycosidases?
- What is a good method to re-purify a glycan or glycopeptide after exoglycosidase treatment?
- Does this enzyme require denaturing conditions to act on glycoproteins?
- How much exoglycosidase should be used?
Tech Tips
- Repeated freeze/thaw cycles may reduce activity. Recommended storage temperature is 4°C.
- Using 1-2 µl is a good starting point for a 1 hr incubation of 1µg of glycoprotein or 100 nM of oligosaccharide.
- Higher enzyme concentrations and longer incubation times may be required for the removal of α1-3 and α1-4 fucose residues from complex N-glycans.
- α1-3,4 Fucosidase can cleave the α1-3 fucose on the outer arm of N-linked glycans but is unable to remove the core α1-3 fucose residue.
- The storage buffer does not contain rHSA; however, we recommend supplementing the reaction with rHSA for optimal enzyme activity.
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- P0769S_L_v1_0041603
- P0769S_L_v1_0041609
- P0769S_L_v1_0041702
- P0769S_L_v1_0041707
- P0769S_L_v1_0051803
- P0769L_v1_10021898
- P0769S_v1_10039254
- P0769S_v1_10042357
- P0769L_v1_10047012
- P0769L_v1_10053308
- P0769S_v1_10053310
- P0769S_v1_10059843
- P0769S_v1_10070626
- P0769S_v1_10082485
- P0769S_v1_10099648
- P0769S_v1_10116028
- P0769S_v1_10144280
- P0769S_v1_10153788
- P0769S_v1_10173173
- P0769S_v1_10181914
- P0769S_v1_10206172
- P0769S_v1_10221055
- P0769S_v1_10227740
- P0769S_v1_10252272
- P0769S_v1_10260957
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.α1-3,4 Fucosidase
GlycoBuffer 1
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.