NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible kit enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.

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5 Reasons to choose NEBuilder HiFi

• Save time – Enjoy simple and fast seamless cloning in as little as 15 minutes.
• Flexibility – Use one system for both "standard-size" cloning and larger gene assembly products, up to 6 fragments.
• Compatible with downstream applications – DNA can be used immediately for transformation or as template for PCR or RCA.
• Adaptable – Adapts easily for multiple DNA manipulations, including site-directed mutagenesis.
• Save money - No licensing fees from NEB for NEBuilder products

 

NEBuilder HiFi DNA assembly offers unique features over other DNA assembly methods, including:

• 3´- and 5´-end mismatch removal
• Bridging of two ds-fragments with a ssDNA oligo
• Improved methods for site-directed mutagenesis

 

Video: Construction of an sgRNA-Cas9 Expression Vector via an ssOligo Bridge. Learn how you can use NEBuilder HiFi to generate an sgRNA-Cas9 expression vector with a single-stranded oligo bridge.

How does NEBuilder HiFi stack up?

Performance data and advantages against NEB Gibson Assembly (links to NEB site)
Performance data and advantages against In-Fusion HD (links to NEB site)
Synthetic Biology/DNA Assembly Selection Chart (links to NEB site)
Comparison of DNA Assembly Reaction Types (links to NEB site)

 

 

Not your average DNA assembly reagent

NEBuilder HiFi DNA Assembly can be used for a variety of DNA assembly methods.

 

Pick your choice of the NEBuilder HiFi products

NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520)

• Includes NEB 5-alpha competent cells
• NEB recommends NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) for routine assemblies of 15 kb or less
• For repetitive sequences, NEB recommends NEB Stable Competent E. coli (NEB #C3040)

NEBuilder HiFi DNA Assembly Bundle for Large Fragments (NEB #E2623)

• Includes NEB 10-beta competent cells
• NEB recommends NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) or NEB 10-beta Electrocompetent E. coli (NEB #C3020) for assemblies larger than 15 kb

NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621)

• Does not include competent cells

 

 

Learn More

• NEBuilder HiFi DNA Assembly – Benefits Over NEB Gibson Assembly (links to NEB site)
• NEBuilder HiFi DNA Assembly – Benefits Over In-Fusion HD (links to NEB site)
• Comparison of DNA Assembly Reaction Types (links to NEB site)

 

Request a Sample of the NEBuilder MasterMix**

 

**This free sample can be requested by laboratory from the Netherlands, Belgium and Luxembourg . No cash or cash equivalent. Offer may not be applied to existing, pending or prior orders. Free samples cannot be requests by current users of the product.