Product Class: Other

pBR322 Vector

Product Introduction

  • View sequence details
  • pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1).
  • The molecule is a double-stranded circle 4,361* base pairs in length (2).
  • pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
  • The molecular weight is 2.83 x 106 daltons. 

*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.

Catalog # Size Concentration
N3033S 50.0 µg 1000 µg/ml
N3033L 250.0 µg 1000 µg/ml

Featured Videos

View Video Library
  • TraditionalCloningWorkflow_720

    Traditional Cloning Workflow

Product Information

Description

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

pBR322 is isolated from E. coli ER2686 (dam +dcm + EcoK M-) by a standard plasmid purification procedure.
pBR322_map
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Copy Number

Medium (10-100)

Origin of Replication

  • pMB1

Lac Repressor on Plasmid

0

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Molecular Weights

Apparent: 2.83 daltons
Theoretical: 2.83 daltons

References

  1. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heynecker, H.L. and Boyer, H.W. (1977). Gene. 2, 95-113.
  2. Watson, N. (1988). Gene. 70, 399-403.

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. How homogeneous is the DNA/ how much is supercoiled?
  2. Are the plasmids cesium purified?
  3. What is the DNA sequence of this product?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In