Suitable for use on both native polyacrylamide and agarose gels.
Formulated with TriDye loading dye, containing 3 different tracking dyes
Easy to identify reference bands
Suitable for 125 to 250 gel lanes
Product Information
TriDye™ Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis.
The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
Recommended gel percentage range: 4% TBE agarose, 10% to 20% TBE polyacrylamide
Migration of TriDye dyes during electrophoresis*
Gel
Xylene Cyanol
Bromophenol Blue
Orange G
4% Agarose
150 bp
20 bp
5 bp
5% Agarose
100 bp
15 bp
5 bp
6% PAGE
400 bp
50 bp
15 bp
10% PAGE
200 bp
30 bp
10 bp
4-20% PAGE
300 bp
20 bp
10 bp
20% PAGE
200 bp
20 bp
10 bp
*These migration rates are approximate and variations may occur based on migration conditions (TAE/TBE buffers, buffer pH, voltage etc.).
TriDye™ Ultra Low Range DNA Ladder visualized on a 4% TBE agarose gel and on a 20% TBE polyacrylamide gel. Mass values are for 0.5 µg/lane. Suggested Load: 5 to 10 µl/lane.
This product is related to the following categories:
Preparation of DNA:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
Loading Recommendations:
For polyacrylamide gels, load 5 µl (0.5 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
For agarose gels, load 10 µl (1 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
Usage Recommendations:
When using the DNA ladder on agarose gels, we recommend using ethidium bromide at 0.5 ug/ml in both the gel and the migration buffer for proper visualization of the smallest DNA fragments. We also recommend that you do not cast gels exceeding 5mm in thickness, as the smaller DNA fragments might diffuse more easily and may be harder to efficiently stain.
We recommend using TBE buffer in the agarose gels and electrophoresis buffers, as TBE buffer allows for a better resolution of the smaller DNA fragments. Fragments below 15 bp might not separate efficiently when using TAE buffer.
We do not recommend using GelRed® or SYBR® dyes as precast gel stains, as these dyes might interfere with the migration of the smallest DNA fragments and result in an abnormal band pattern. We recommend using these dyes as post-electrophoresis stains only. Optimization might be required for proper visualization of all DNA fragments. Intensity of some bands might vary.
If using wide combs (10 mm) for agarose gel electrophoresis, we recommend loading 15 ul (1.5 ug) of TriDye™ Ultra Low Range DNA Ladder per gel lane for efficient visualization of the smallest DNA fragments.
For optimal visualization of all the bands, we recommend exposing the gel to UV without any casting tray whenever possible. Even UV-transparent trays can obscure DNA fragments under UV exposure.
Usage Notes:
When using the DNA ladder on agarose gels, we recommend using ethidium bromide at 0.5 ug/ml in both the gel and the migration buffer for proper visualization of the smallest DNA fragments. We also recommend that you do not cast gels exceeding 5mm in thickness, as the smaller DNA fragments might diffuse more easily and may be harder to efficiently stain.
We recommend using TBE buffer in the agarose gels and electrophoresis buffers, as TBE buffer allows for a better resolution of the smaller DNA fragments. Fragments below 15 bp might not separate efficiently when using TAE buffer.
We do not recommend using GelRed® or SYBR® dyes as precast gel stains, as these dyes might interfere with the migration of the smallest DNA fragments and result in an abnormal band pattern. We recommend using these dyes as post-electrophoresis stains only. Optimization might be required for proper visualization of all DNA fragments. Intensity of some bands might vary.
If using wide combs (10 mm) for agarose gel electrophoresis, we recommend loading 15 ul (1.5 ug) of TriDye™ Ultra Low Range DNA Ladder per gel lane for efficient visualization of the smallest DNA fragments.
For optimal visualization of all the bands, we recommend exposing the gel to UV without any casting tray whenever possible. Even UV-transparent trays can obscure DNA fragments under UV exposure.
References
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.
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