Product Class: Other

dsRNA Ladder

Product Introduction

  • Size range: 21 - 500 base pairs
  • Compatible with non-denaturing polyacrylamide and agarose gels
  • Convenient reference band at 80 base pairs

 

Catalog # Size Concentration
N0363S 25.0 gel lanes

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Product Information

Description

The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.

This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.

 

 


N0363_v1_000009
2 µl/lane. 6% Polyacrylamide gel.

3µl/lane. 2% TBE agarose gel.
This product is related to the following categories:
RNA Markers & Ladders Products,
RNA Markers and Ladders,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment bp
1 500
2 300
3 150
4 80
5 50
6 30
7 21

Effective Size Range

21bp to 500bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Product Notes

  1. This marker was not designed for precise quantification of dsRNA mass.
  2. Double-stranded RNA is sensitive to degradation by ribonucleases, even though it is more resistant than single-stranded RNA.

    To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse throughly with RNase-free water.

    Excessively long run times or high voltage can cause degradation of the bands on the gel. We recommend loading 2-3 µl of dsRNA Ladder, a running voltage of 4-10 V/cm , adding ethidium bromide to gels and running buffer at a final concentration of 0.5 µg/ml to effectively stain the bands during agarose gel electrophoresis.

References

  1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), (p. 7.43?7.45),
  2. Liu, Y-C. and Chou, Y-C. (1990). Biotechniques . 9,
  3. Dong Ma unpublished observation.

FAQs & Troubleshooting

FAQs

  1. What types of gels are recommended for ssRNA and dsRNA ladders? (N0363, N0362, N0364)
  2. What types of gel and staining dyes can I use for NEB’s dsRNA Ladder (N0363S)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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