Product Class: Other

NEBNext® Q5U® Master Mix

Product Introduction

NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

  • Optimized for high yields in uracil-containing NGS library amplification
  • Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
Catalog # Size Concentration
M0597S 50.0 reactions 2 X
M0597L 250.0 reactions 2 X

Product Information

Description

NEBNext Q5U Master Mix is optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
The polymerase component of the master mix, Q5U, is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. The NEBNext Q5U Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. 

NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.
This product is related to the following categories:
Epigenetic Analysis Products,
Epigenetics Products,
Q5® High-Fidelity DNA Polymerases Products,
PCR, qPCR & Amplification Technologies Products,
This product can be used in the following applications:
Polymerases for NGS Library Preparation,
DNA Methylation Analysis,
Bisulfite Conversion,
Epigenetics,
Specialty PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Reaction Setup NEBNext® Q5U® Master Mix (NEB #M0597)

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. Can NEBNext Q5U Master Mix be used to amplify templates containing uracils?
  2. Is the NEBNext Q5U Master Mix a hot start formulation?
  3. What cycling conditions should I use for the NEBNext Q5U Master Mix?
  4. How many cycles of PCR should I perform with the NEBNext Q5U Master Mix?
  5. Are the DNA products produced by the NEBNext Q5U Master Mix blunt-ended or do they have a single base 3´ overhang?
  6. Will NEBNext Ultra II Q5 Master Mix incorporate dUTPs?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is licensed from University of Newcastle Upon Tyne under EPO Patent No. 1463809B1 and Australian Patent No. AU2003226543. 

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See US Patent Nos. 7,947,447; 8,404,830; 8,975,388 and their foreign counterparts). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based NEBNEXT Q5U MasterMix for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based NEBNEXT Q5U MasterMix requires a license from New England Biolabs, Inc. 

For additional information or to inquire about commercial use, please contact busdev@neb.com.

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