Product Class: Other

Exonuclease VII
NEBU cloned at NEB recombinant 37 95 Heat

Product Introduction

  • DNA specific exonuclease
  • Cleaves linear single-stranded DNA in both the 3' to 5' and 5' to 3' directions
  • Preferred substrate is linear single-stranded DNA
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease VII is ideal for:

  • Removal of primers with or without 3' or 5' terminal phosphorothioate bonds
  • Mapping positions of introns in genomic DNA
  • Removal of single-stranded DNA, leaving behind the double-stranded DNA in a sample
Catalog # Size Concentration
M0379S 200.0 units 10000 units/ml
M0379L 1000.0 units 10000 units/ml

Product Information

Description

Exonuclease VII, (Exo VII) derived from E. coli, cleaves single-stranded DNA (ssDNA) from both 5´→3´ and 3´→5´ direction. This enzyme is not active on linear or circular dsDNA (1,2). It is useful for removal of single stranded oligonucleotide primers (3) from a completed PCR reaction when different primers are required for subsequent PCR reactions. Digestion of ssDNA by Exonuclease VII is metal-independent.

Product Source

An E. coli strain that carries cloned Exonuclease VII (XseA and XseB) genes from E. coli.
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0379S     -20    
      Exonuclease VII M0379SVIAL -20 1 x 0.02 ml 10,000 units/ml
      Exonuclease VII Reaction Buffer B0379SVIAL -20 1 x 1.5 ml 5 X
  • M0379L     -20    
      Exonuclease VII M0379LVIAL -20 1 x 0.1 ml 10,000 units/ml
      Exonuclease VII Reaction Buffer B0379SVIAL -20 1 x 1.5 ml 5 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C.

Reaction Conditions

1X Exonuclease VII Reaction Buffer
Incubate at 37°C

1X Exonuclease VII Reaction Buffer
50 mM Tris-HCl
50 mM Sodium Phosphate
8 mM EDTA
10 mM 2-mercaptoethanol
(pH 8 @ 25°C)

Storage Buffer

50% Glycerol
50 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
0.1 M NaCl
0.1% Triton® X-100
pH 7.5 @ 25°C

Heat Inactivation

95°C for 10 minutes

Unit Assay Conditions

1X Exonuclease VII Reaction Buffer containing 0.15 mM sonicated single-stranded [3H] E. coli DNA.

Application Features

  • Removal of single-stranded oligonucleotide primers after PCR (3)
  • Removal of terminal phosphorothioated ss-oligonucleotide primers after PCR
  • Mapping positions of introns in genomic DNA (4)
  • Removal of single-stranded DNA from dsDNA

References

  1. Chase, et al. (1974). J. Biol. Chem. 249, 4545-4552.
  2. Chase, et al. (1974). J. Biol. Chem. 249, 4553-4561.
  3. Li, H. et al. (1991). Nucl. Acids Res. 19, 3139-3141.
  4. Berk, A.J. et al. (1978). Proc. Natl. Acad. Sci. USA. 75, 1274-1278.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Exonuclease VII (M0379)

Tools & Resources

Selection Charts

Web Tools

Research Posters

FAQs & Troubleshooting

FAQs

  1. What is the activity of Exonuclease VII in the following buffers?
  2. Will Exonuclease VII work in a PCR buffer?
  3. What is the molecular weight of the Exonuclease VII?
  4. Is Exonuclease VII a tagged protein?
  5. Does Exonuclease VII cut RNA?
  6. Can DNA be blunted using Exonuclease VII?
  7. What is the difference between Exonuclease VII and Exonuclease I (NEB# M0293)?
  8. How to remove primers from a PCR reaction?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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